Identification of the re-programming function domain existing in ribosome

2016 Fiscal Year Final Research Report

• Project/Area Number: 16K14741
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kumamoto University
• Principal Investigator: OHTA KUNIMASA 熊本大学, 大学院生命科学研究部(医), 准教授 (90244128)
• Research Collaborator: ITO NAOFUMI 熊本大学, 大学院生命科学研究部, 研究員 (60732716)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Keywords: リボソーム / 幹細胞 / リプログラミング

Outline of Final Research Achievements

I homogenized the lactic acid bacteria (LAB) ,separated them to each fraction by chromatography, and performed the cell cluster formation assay. I found that a single fraction has cell cluster formation activity and it contained some proteins derived from the ribosome. Then, I purified the ribosome from LAB by the ultra centrifugation, and performed the same cell cluster formation assay. Interestingly, it showed the cell clusters formation. From these results, I confirmed that the reprogramming material from LAB is ribosome (Ito et al., revised).

Free Research Field

生物学