2017 Fiscal Year Final Research Report
Analysis of salicylic acid response in a single plant cell using microfluidics
| Project/Area Number |
16K14750
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TAKINOUE Masahiro 東京工業大学, 大学院総合理工学研究科, 准教授 (20511249)
KAMIYA Koki 神奈川科学技術アカデミー, 人工細胞膜システムグループ, 研究員 (70612315)
TABATA Kazuhito 東京大学, 大学院工学研究科, 准教授 (50403001)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 植物免疫 / レポーター植物 / MEMS / サリチル酸 |
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| Outline of Final Research Achievements |
To understand plant cellular response against salicylic acid (SA), a hormone required for plant immune response, we aimed to establish a system to analyze the SA response at the single cell level using microfluidic devices and the protoplast derived from Arabidopsis transgenic plants carrying YFP-NLS under the control of the SA-responsive PR1 promoter (pPR1 plants). While we established the 2nd generation pPR1 plants that should enable us to perform genetic analysis as well as highly sensitive imaging, we also conditioned microfluidics-based single cell analysis using the 1st generation pPR1 plants with YFP-NLS as a reporter. In this study, the protoplast from the 1st generation pPR1 plants did not show any YFP signal in response to SA treatment, although the leaf cells of the same pPR1 plant exhibited YFP signal clearly after SA treatment. We are currently investigating the reason of this unresponsiveness only found in protoplasts.
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| Free Research Field |
植物病理学
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