Super-resolution live-imaging of RNA molecules

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14773
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Masui Osamu 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 研究員 (30579305)
• Research Collaborator: KEPTEN Eldad Curie Institute
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: エピジェネティクス / non-coding RNA / X染色体不活性化 / Xist / 超解像顕微鏡 / ライブイメージング

Outline of Final Research Achievements

In this research project, we aimed to develop a new microscopic technology, super-resolution live-imaging, by combining 3D-SIM and time-lapse analysis. We have succeeded in establishing the basis of super-resolution live-imaging with 3D-SIM in this study. We have also published 2 scientific papers as relating researches. (1) We have developed a new methodology to visualize RNA molecules as GFP signals (Methods Mol Biol, in press). (2) We have clarified the spatial relationship between Xist RNA and PCGF3/5-PRC1 protein complex, a subcomplex of PRC1 (Science, 2017, 356, 1081-1084).

Free Research Field

エピジェネティクス