Regulation of somatic hypermutation on the immunoglobulin gene by novel functions of an RNA splicing factor

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14783
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Okayama University
• Principal Investigator: KANAYAMA Naoki 岡山大学, 自然科学研究科, 准教授 (70304334)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: SRタンパク質 / スプライシング / AID / DT40

Outline of Final Research Achievements

The affinity of antibodies is improved by somatic hypermutation on the immunoglobulin variable region gene. Although it has been shown that AID is essential for somatic hypermutation, it remains unclear how the AID-dependent mutation machinery works. In this study, functions of SRSF1-3, which is an isoform of the RNA splicing factor SRSF1 and essential for somatic hypermutation, were analyzed using a hypermutating B cell line. Results revealed that SRSF1-3 forms a protein complex with AID, and promotes somatic hypermutation through a mechanism linked to the C-terminal region of AID.

Free Research Field

細胞工学、免疫学