Verification of the hypothesis of the 'Tripartite Symbiosis' among Flower-Yeast and insect, by using metagenomics and chemical ecology.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14809
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ecology/Environment
• Research Institution: Gifu University
• Principal Investigator: Suzuki Tohru 岐阜大学, 応用生物科学部, 教授 (20235972)
• Co-Investigator(Kenkyū-buntansha): 岡本 朋子 岐阜大学, 応用生物科学部, 助教 (50588150)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: insect / yeast / flower / metagenome / chemical ecology

Outline of Final Research Achievements

The aromatic components produced by yeasts should play important roles in insects induction. We hypothesized that 'a tripartite symbiosis' has been established among flower - yeast - insects. In this study, we tried to verify this hypothesis from the standpoints of chemical symbiosis and metagenome analysis.
For yeast, rRNA D1 - D2 region, Saccharomyces cerevisiae, six microsatellites, Rubisco gene rbcL for flowers, insects for mitochondrial cytochrome C oxidase subunit I (COI) gene as the target, multiplex PCR Established the method. Simultaneous identification of yeasts, plants, insects was made possible from samples of 384 samples of plants at the same time.

Free Research Field

生物学