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2018 Fiscal Year Final Research Report

Functional annotation of unknown sequences through gene expression by substrate induction

Research Project

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Project/Area Number 16K14817
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Ecology/Environment
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

MORONO Yuki  国立研究開発法人海洋研究開発機構, 高知コア研究所, 主任研究員 (30421845)

Project Period (FY) 2016-04-01 – 2019-03-31
Keywords海底下生命圏 / 基質誘導 / 遺伝子発現
Outline of Final Research Achievements

In this study, we explored unknown function buried in genomic DNA obtained from subseafloor biosphere, one of the most unexplored microbial habitat on Earth, by applying Substrate Induced Gene EXpression (SIGEX) method. We extracted DNA from various subseafloor sediment samples and established procedure to construct genomic library with sequence length in average of 1700 bp and millions of clonal variations. In parallel, we also obtained metagenomic sequence information to further acquire functional information of DNA fragment. This SIGEX approach can be useful technique to decipher the gene functional information even for unannotatable sequences by metagenomic approach.

Free Research Field

地球微生物学

Academic Significance and Societal Importance of the Research Achievements

DNAやRNAの配列を高速に解析する技術が日進月歩で発展している。一方で、膨大に得られる塩基配列情報がどのような機能を持つか、その意味付け(アノテーション)はデータベースでの相動性検索によるところが大きい。本研究では基質誘導によって遺伝子のスイッチがオンになる、発現、という現象を基に未知DNA配列の機能を推定しようとするアプローチである。実際に得られた配列についてデータベース検索を行ったところ、用いた基質との関連情報が存在しないものばかりであった。

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Published: 2020-03-30  

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