Development of technology for leaf color-alteration by the control of gene expression using tissue layer- or floral differentiation period-specific promoter

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14849
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Horticultural science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: suzuki sakae 東京農工大学, (連合)農学研究科(研究院), 講師 (80397017)
• Research Collaborator: ITO Risa ; EBE Yuriko ; KOZIMA Saeka ; SUGANOMATA Masumi ; YAMAMOTO Kaho
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 葉色改変 / 組織特異的 / アントシアニン / カロテノイド / 花芽分化 / キメラ

Outline of Final Research Achievements

In the present study, we try to develop a technology for leaf color-alteration by the control of gene expression using tissue layer- or floral differentiation period-specific promoter.
In the Tobacco plants, when the vectors of a combination with AtPIP2;1 or AtIAMT1 promoter and Atpap1 or NtPDS-RNAi gene were introduced, the color of leaf vein in transgenic plants was changed to red (Atpap1) or white (NtPDS-RNAi). Furthermore, the same results were observed in the plants of self-pollinated progenies. When the vectors of a combination with AtFT promoter and Atpap1 gene were introduced, in contrast, no color-alteration was observed in transgenic plants.

Free Research Field

園芸学