2017 Fiscal Year Final Research Report
Comprehensive analysis of full length cDNA and alternative splicing for the genes related to fruit development and ripening
| Project/Area Number |
16K14854
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Horticultural science
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
門田 有希 岡山大学, 環境生命科学研究科, 准教授 (30646089)
赤木 剛士 京都大学, 農学研究科, 助教 (50611919)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | Iso-seq |
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| Outline of Final Research Achievements |
3rd generation sequences promotes not only genome sequencing but also transcript analysis. Iso-seq analysis using PacBio are very useful method for reading full length cDNA and detecting alternative splicing. In this analysis, we carried out Iso-seq analysis for melon fruits. We used three different methods for construction of full length cDNA library. The libraries were sequenced by PacBio sequel and Nanopore MinION. Isoforms were mapped on the reference genome of melon and compared them. PacBio and Nanopore isoforms fell into ~ 6,500 and 12,000 loci respectively. 509 loci of them were located at intergenic region and predicted novel loci.
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| Free Research Field |
園芸学
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