Development of novel microbial oxidation-reduction/electron transferring function acting under high temperature and high alkaline conditions

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14884
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Kyoto University
• Principal Investigator: Ogawa Jun 京都大学, 農学研究科, 教授 (70281102)
• Co-Investigator(Kenkyū-buntansha): 櫻谷 英治 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 教授 (10362427) ; 安藤 晃規 京都大学, 農学研究科, 助教 (10537765) ; 日比 慎 京都大学, 農学研究科, 特定准教授 (30432347)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 電子伝達 / インジゴ / 藍染

Outline of Final Research Achievements

From indigo dyeing process of high temperature/high alkali, isolation of microorganisms with potential oxidation-reduction reactions useful as tools such as nitrification denitrification and enzyme batteries was carried out. Then, the evaluation and analysis of the selected redox function were performed. The quantification method of indigo reducing activity was constructed and indigo reducing bacteria were screened. Ten indigo reducing bacteria were obtained from 154 strains isolated from indigo dyeing sample. Selected Alkalibacterium sp. SH2-1 strain had higher activity than known indigo reducing bacteria. SH2-1 reduced indigo directly without mediators with the presence of glucose as an electron donner. Some anthraquinon derivatives including emodin, a natural anthraquinon in the leaf of Persicaria tinctoria, were found to act as mediator for the indigo reduction by SH2-1. Some microorganisms with P. tinctoria degrading activity were also isolated.

Free Research Field

応用微生物学