2017 Fiscal Year Final Research Report
Establishment of method for injection of heterologous genomic DNA into bacterial giant cells
| Project/Area Number |
16K14891
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Toyama Prefectural University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | スフェロプラスト / 巨大化細胞 / マイクロマニュピレーション操作 / 金属イオン / Lelliottia amnigena / 光合成細菌 / Deinococcus grandis / マイクロインジェクション |
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| Outline of Final Research Achievements |
Injection of foreign DNA into bacterial cells has limitation in size and it is impossible to inject chromosome size DNA (> 1 Mbp). Even if genome of bacteria is designed and DNA can be synthesized based on its design drawing, injection of the DNA into the cell is impossible. So, we aimed to establish a method to inject foreign long DNA by piercing the microchip into the cell. In order to achieve the objective, it was necessary to enlarge the bacterial cells of 1-2 μm up to >15 μm. We succeeded in enlarging several bacterial cells so far and carried out micromanipulation operation. Particularly in the giant cells of Lelliottia amnigena, noteworthy results could be raised.
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| Free Research Field |
応用微生物学
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