2017 Fiscal Year Final Research Report
Does caffene function as rhizosphere signal?
| Project/Area Number |
16K14901
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Keywords | カフェイン / 根圏微生物 |
|---|
| Outline of Final Research Achievements |
In this study transgenic tomato plants expressing genes involved in the caffeine synthesis was generated. These transgenic plants synthesized caffeine and secreted as well. The effect of caffeine to the rhizosphere microbes was analyzed. More than 20 strains were isolated from rhizosphere soils of coffee plants. No of these bacteria showed caffeine metabolizing activities. The effect of caffeine to these bacterial was tested in vitro. Caffeine synthesis and secretion were also analyzed using the seedlings of coffee plants. From the genome analysis and gene expression analysis several candidate genes involved in the caffeine secretion were isolated.
|
| Free Research Field |
植物生化学
|
