2017 Fiscal Year Final Research Report
Development a novel method to produce a single protein in yeast
| Project/Area Number |
16K14906
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Osaka City University |
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Principal Investigator |
Yamaguchi Yoshihiro 大阪市立大学, 複合先端研究機構, 特任准教授(テニュアトラック) (00737009)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | SPP system / MqsR / MazF / yeast |
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| Outline of Final Research Achievements |
In this study, our goal is to develop SPP system in Saccharomyces cerevisiae. To archive it, I first construct two plasmids. One is to express sequence-specific endoribonuclease and another is to express target protein.
We constructed the two plasmids to express MazF or MqsR and a target protein, respectively. We tried may conditions however we could not develop yeast SPP system.
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| Free Research Field |
応用微生物
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