Elucidation and utilization of biosynthetic pathway not involving known isopentenyl diphosphate isomerase homologs

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K14911
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bioorganic chemistry
• Research Institution: Niigata University
• Principal Investigator: Sato Tsutomu 新潟大学, 自然科学系, 准教授 (80334655)
• Research Collaborator: KUZUYAMA Tomohisa ; UMENO Daisuke
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 生合成 / イソプレノイド / テルペノイド

Outline of Final Research Achievements

Farnesyl diphosphate synthetase synthesized farnesyl diphosphate using only isopentenyl diphosphate as a substrate. This result suggested that it also has isopentenyl diphosphate isomerase activity. That is, it turned out that it is a bifunctional enzyme. Currently, we are conducting research aimed at elucidating the catalytic mechanism.

Free Research Field

生物有機化学

Academic Significance and Societal Importance of the Research Achievements

新型酵素や二機能性酵素が関与するイソプレノイド生合成の新規基幹経路や新しい触媒機構の解明は、生物有機化学の基礎研究として重要なだけでなく、薬剤・香料・バイオ燃料などのバイオ生産や医薬開発などの応用に新しい展開をもたらすものと期待される。