Collective activation of silent biosynthetic pathways for bioactive compounds by small diffusible signaling molecules

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14915
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bioorganic chemistry
• Research Institution: Hiroshima University
• Principal Investigator: ARAKAWA Kenji 広島大学, 先端物質科学研究科, 准教授 (80346527)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: シグナル分子 / 二次代謝生合成 / 放線菌 / ゲノムマイニング / P450 / デヒドロゲナーゼ

Outline of Final Research Achievements

Secondary metabolite production in Streptomyces is strictly controlled by signaling moleclues. We analyzed structural diversity, biosynthesis and biological activity of the signaling molecules SRBs that induce antibiotic production in Streptomyces rochei. The followings are short summary in this project.
(1) Around 80 Streptomyces strains showed no ability to induce antibiotic production in S. rochei, suggesting their signaling molecules are different from SRB-type molecules. (2) P450 monooxygenase SrrO is responsible to oxidize C-6’ methylene group during SRB biosynthesis. (3) Exogenous addition of SRBs caused alteration of the metabolic profiles among some Streptomyces strains.

Free Research Field

生物有機化学