Chemometric approach to matrix effect suppression in LC-ESI-MS/MS

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K14931
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Osaka City Institute of Public Health and Environmental Sciences
• Principal Investigator: Daichi Asakawa 大阪市立環境科学研究センター, その他部局等, 研究員 (80470251)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 液体クロマトグラフ質量分析計 / マトリクス効果 / 計量化学 / 食品分析

Outline of Final Research Achievements

We conducted a novel approach to the suppression of the matrix effect in liquid chromatograph mass spectrometers, which causes measurement inhibition when performing simultaneous analysis of residual pesticides and veterinary drugs in foods. We developed a new analysis method and established a method to convert various detector data into matrix data that can be analyzed by multivariate analysis. The present study showed that, in addition to the presence of contaminating components that inhibit ionization, changes in the mobile phase composition and the ionization voltage of the target compound also have a significant effect on the matrix effect. It is necessary to consider a matrix effect suppression method that takes these factors into consideration.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

異なる検出器データを統合して多変量解析を行うことで、従来とは異なる知見が得られることが示された。本研究の進展によって液体クロマトグラフ質量分析法のマトリクス効果が抑制されることで、食品中の残留農薬や動物用医薬品の分析精度が向上し、安全な食品の流通に貢献することが期待できる。また、マトリクス効果を抑制して信頼性の高い測定値を得ることは、食品科学のみでなく多くの研究分野での共通課題であるため、他分野への貢献も期待できる。