2017 Fiscal Year Final Research Report
Analysis of ion channel functioning in the spirochete flagella by genetically chimeric technique
| Project/Area Number |
16K15036
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Research Collaborator |
MORIMOTO Yusuke 九州工業大学, 生命情報工学科, 助教
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 細菌べん毛 / スピロヘータ / 固定子 / レプトスピラ / イオンチャネル / 分子モーター |
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| Outline of Final Research Achievements |
Bacterial motility is an essential virulence factor, and understanding its mechanism leads to development of technology for phylaxis. We focused on periplasmic flagella of spirochetes and attempted to clarify their rotation mechanism by constructing chimeric flagella composed of spirochete stator units and a flagellar rotor and filament from externally flagellated bacteria. We introduced a plasmid coding stator genes of the spirochete Leptospira into a Salmonella strain lacking stator genes. Although stator proteins, MotA and MotB, seemed to be expressed in Salmonella cells and were probably incorporated to the cell membrane, we could not observe flagellar rotation. Replacement of the peptidoglycan biding domain in MotB of Salmonella with that of Salmonella also did not work. These results suggests that modifications of ion channel or parts responsible for torque generation could be required to make chimeric Salmonella flagella rotated by Leptospira stator units.
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| Free Research Field |
生物物理学、細菌学
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