2017 Fiscal Year Final Research Report
Overproduction of functional membrane proteins by improvement of integration factors of E. coli
| Project/Area Number |
16K15083
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Iwate University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SHIMAMOTO Keiko 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 主幹研究員 (70235638)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 膜タンパク質 / タンパク質膜挿入 / MPIase / 糖脂質酵素 |
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| Outline of Final Research Achievements |
Protein integration into the cytoplasmic membranes of E. coli requires a glycolipid termed as MPIase in addition to a series of proteinaceous factors such as SRP and SecYEG. We found that CdsA that is known to be involved in phospholipid biosynthesis, is also involved in MPIase biosynthesis. CdsA is a rate-limiting enzyme for MPIase biosynthesis, since CdsA depletion causes MPIase depletion and CdsA overproduction causes MPIase overproduction. We constructed a strain, in which not only MPIase, but also SecYEG, SRP and YidC were overproduced. By means of this strain, we achieved overproduction of several membrane proteins including F0c. F0c.
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| Free Research Field |
生化学
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