2017 Fiscal Year Final Research Report
Production of functional RNA from methanol using high transcription potential of methylotrophic yeasts
| Project/Area Number |
16K15089
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SAKAI Yasuyoshi 京都大学, 大学院農学研究科, 教授 (60202082)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | メタノール酵母 / RNA顆粒 / mRNA |
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| Outline of Final Research Achievements |
For the purpose of development of production system for functional RNA from methanol using methylotrophic yeasts which have strong methanol-inducible gene expression and can grow at high cell density, we investigated formation and dynamics of RNA granules, P-bodies and stress granules (SGs), which are involved in storage and degradation of mRNA. We also analyzed intracellular dynamics of mRNAs which are highly transcribed during growth on methanol. Formation of P-bodies was observed under various stress conditions and during growth on methanol, but SGs were observed only under high-temperature condition. Furthermore, we revealed that a stress responsive regulator Hog1 is recruited to SGs under high-temperature condition.
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| Free Research Field |
応用微生物学
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