2017 Fiscal Year Final Research Report
Development of new culture system using emulsion drop for the environmental BNC microbiome
| Project/Area Number |
16K15091
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | エマルジョンドロップレット / 微小培養 / 環境微生物 / 難培養性微生物 / ミリオンスケール |
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| Outline of Final Research Achievements |
We examined the application possibility of emulsion drop culture system for environmental BNC (viable but non-culturable) microbiome. First, we established the method to produce the emulsion drop stably and then showed the growth of E. coli and yeast in emulsion drop. Next, when the microbiome flora in the emulsion drop in which the environmental soil microbiome were enclosed were analyzed by 16S rDNA NGS analysis, the growth of environmental BNC (viable but non-culturable) microbiome were detected. This indicated the emulsion drop system is new technology for analyzing the environmental microbiome.
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| Free Research Field |
分子生物学
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