2017 Fiscal Year Final Research Report
The AID technology for ES cells and mice
| Project/Area Number |
16K15095
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
Kanemaki Masato 国立遺伝学研究所, 分子遺伝研究系, 教授 (20444507)
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| Research Collaborator |
HIRATANI Ichiro 理化学研究所, 多細胞システム形成研究センター, グループリーダー (40583753)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 発現制御 / デグロン |
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| Outline of Final Research Achievements |
In order to use auxin-inducible degron in mouse ES cells, it is required to introduce the ubiquitin ligase subunit, OsTIR1. We constructed a vector for introducing the OsTIR1 gene at the ROSA26 locus. We are currently making OsTIR1 expressing cells in E14TG2a background. In other ES lines, we successfully tagged genes with the degron to make conditional mutants.
To use in mice, we synthesized auxin analogs that might have low toxicity. We identified couples of analogs that showed a similar degradation-inducing activity to the natural auxin, IAA.
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| Free Research Field |
細胞工学
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