Analyses of the oligomers of amyloid beta by NMR/MRI and protein science

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15110
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Kumamoto University
• Principal Investigator: TERASAWA HIROAKI 熊本大学, 大学院生命科学研究部(薬), 教授 (10300956)
• Co-Investigator(Renkei-kenkyūsha): YOSHINAGA Sosuke 熊本大学, 大学院生命科学研究部(薬), 講師 (00448515) ; TAKEDA Mitsuhiro 熊本大学, 大学院生命科学研究部(薬), 助教 (90508558) ; SAIDO Takaomi 国立研究開発法人理化学研究所, 脳科学総合研究センター, 教授 (80205690)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 認知症 / アミロイドβ / 神経毒性オリゴマー

Outline of Final Research Achievements

Amyloid beta (Abeta) is often bound　in　the　brain　of　patients　with　Alzheimer　disease　as　insoluble　aggregates.　It is generally asumed that soluble ogligomer that occur before the aggregates exerts strong neurotoxicty. In this study, we explored the interaction mode of two monomers comprisng the Abeta dimer that is the minimal component of Abeta oligoemrs, using NMR, photo-induced cross coupling and mass analyses. The results showed that the two monomers aling in both parallel and anti-parallel manner in a 2:1 ratio, indicating that both the two forms exist, but the parallel one is more preferred.

Free Research Field

構造生物学