2017 Fiscal Year Final Research Report
Exhaustive identification of mutations conferring drug resistance in influenza virus NA
| Project/Area Number |
16K15151
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Environmental and hygienic pharmacy
|
|---|
| Research Institution | University of Shizuoka |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
IKEDA Kiyoshi 広島国際大学, 薬学部, 教授 (40168125)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Keywords | インフルエンザウイルス / 抗インフルエンザ薬 / 薬剤耐性 / シアリダーゼ / ノイラミニダーゼ / イメージング / 感染細胞 / 分離 |
|---|
| Outline of Final Research Achievements |
Influenza A and B viruses and their infected cells express an enzyme “sialidase”. We developed and commercialized a probe “BTP3-Neu5Ac” for fluorescence imaging of sialidase activity. We developed a novel technique for selective fluorescence cell imaging of drug-resistant virus-infected cells and for high-efficiency isolation of drug-resistant viruses, as application of BTP3-Neu5Ac. For the purpose of exhaustive identification of sialidase mutations conferring drug resistance, we tried viral sialidase expression in yeast but did not succeed. As an alternative way, we developed an easy and speedy method for detecting drug-resistant virus by use of BTP3-Neu5Ac. This method is expected to enable efficient detection of drug-resistant viruses and to contribute to progress of research on drug resistance mechanism.
|
| Free Research Field |
医歯薬学
|
