2017 Fiscal Year Final Research Report
Development of a new transgenic mouse model as a platform for the imaging-based drug discovery and development
| Project/Area Number |
16K15201
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
伊藤 教道 名古屋大学, 医学部附属病院, 特任助教 (30726310)
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| Co-Investigator(Renkei-kenkyūsha) |
NAGAI Taku 名古屋大学, 医学部附属病院, 准教授 (10377426)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | イメージング創薬 / Npas4 / トランスジェニックマウス / Homer1a / キンドリング |
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| Outline of Final Research Achievements |
In 2016, to regulate the expression of target genes in a neuronal activity-dependent manner, we created a new transgenic mice line (NPAS4-tTA mice) in which the expression of tTA gene is regulated under the control of Npas4 promoter. We obtained the double transgenic mice following mating with tet0-ChR2-EYFP transgenic mice as a reporter. The double transgenic mice exhibited a high background expression of EYFP in the brain with or without pentylentetrazole (PTZ) treatment.
In 2017, we prepared cyclic AMP response element (CRE)-luciferase expression system using AAV vector. We observed a significant increase in chemiluminescent after cocaine treatment in vivo in the brain of mice expressing the CRE-luciferase gene. As to the functional analysis of target genes of Npas4, we demonstrated that Npas4-Homer1a signal played a role in the regulation of homeostatic scaling in the hippocampus.
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| Free Research Field |
薬理学
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