Mechanism analysis of neural stem cell maintenance using an in vitro neural stem cell niche ECM model

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15209
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Yamagata University
• Principal Investigator: Takashi Hoshiba 山形大学, 大学院理工学研究科, 准教授 (00469769)
• Research Collaborator: Chen Guoping 物質・材料研究機構, 生体組織再生材料グループ, グループリーダー ; Ito Yoshihiro 理化学研究所, 伊藤ナノ医工学研究室, 主任研究員
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 細胞外マトリックス / 神経幹細胞 / 幹細胞ニッチェ / 脱細胞化

Outline of Final Research Achievements

Neural stem cells (NSCs) maintain their stemness in a special architecture called as "stem cell niche". Particularly, stem cell niche for NSCs contains a specific ECM structure called as "fractones" and fractones contribute to NSC maintenance. However, it remains unclear how fractones maintain NSC stemness. Here, an in vitro fractones model is prepared by the culture of NSCs and decellularization techniques.
Prepared fractones model possessed cell adhesion ability and cell proliferation ability. Also, the model suppressed NSC differentiation into neural cells.

Free Research Field

生体材料