2017 Fiscal Year Final Research Report
Development of a novel method for the identification of ncRNA-binding proteins
| Project/Area Number |
16K15212
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Research Collaborator |
KUBOTA YUJI 東京大学, 医科学研究所, 助教 (70614973)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 非コードRNA / 蛋白質 |
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| Outline of Final Research Achievements |
Non-coding (nc) RNAs are functional RNA molecules that are transcribed from genomic DNA. Recent studies have demonstrated that various ncRNAs (e.g., miRNAs and lncRNAs) play important roles in the regulation of diverse cellular functions such as cell proliferation, and apoptosis through interacting with specific RNAs or proteins. Furthermore, accumulating evidence showed that abnormal expression of ncRNAs is involved in the etiology of human diseases including cancers. However, the functions of individual ncRNAs remain largely unknown. In this study, we aimed to establish a novel method to identify proteins that selectively bind to a specific ncRNA. For this purpose, we performed several pilot studies using a representative lncRNA, whose binding partners have already been identified. By constructing various expression vectors and optimizing experimental conditions, we succeeded in establishing the novel method. This method will greatly advance our understanding of ncRNAs.
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| Free Research Field |
分子細胞生物学
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