Development of a new optogenetic tools and its application toward the understanding of the mechanism of signal transduction

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15225
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: Murakoshi Hideji 生理学研究所, 脳機能計測・支援センター, 准教授 (90608142)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: シグナル伝達 / LOV2 / 蛍光タンパク質

Outline of Final Research Achievements

In this project, we aimed to develop optogenetic tools to selectively activate a specific pathway of biochemical signaling in synapses. These tools enable us to identify the essential pathways and signaling molecules required for the induction of long-term potentiation. We especially focused on developing a new imaging probe that can be used in combination of light sensitive proteins. As a result of our research, we successfully developed a new probe optimized for 2-photon Fluorescence Lifetime Imaging (2pFLIM) based Forster resonance energy transfer (FRET).

Free Research Field

生物物理、細胞生物、神経科学