Identification of genetic regulators for intracellular aggregation by genome-wide CRISPR screening

2016 Fiscal Year Final Research Report

• Project/Area Number: 16K15229
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Tomita Taisuke 東京大学, 大学院薬学系研究科, 教授 (30292957)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Keywords: 認知症 / 凝集タンパク質 / CRIPSR/Cas9 / タウ / 脳神経疾患

Outline of Final Research Achievements

Intracellular aggregation of tau protein is one of pathological hallmarks in the brains of dementia patients. Identification of regulators for the generation or clearance of the intracellular tau aggregates is important for the development of therapeutic strategies against dementia. To screen the genetic regulators for tau aggregates in genome-wide manner by CRIPSR/Cas9 system, we established the culture cell system with intracellular tau aggregation. These aggregates showed similar biochemical characters with that derived from tau transgenic animal brain. Also qualitative and quantitative changes of intracellular aggregation can be analyzed by FACS. We will perform genome-wide screening of the genetic regulators by genome editing.

Free Research Field

病態生化学