2017 Fiscal Year Final Research Report
Development of combinational therapy of non-thermal plasma and carbon nanotubes
| Project/Area Number |
16K15257
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Research Collaborator |
KIKKAWA Fumitaka
KAJIYAMA Hiroaki
HORI Masaru
TANAKA Hiromasa
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 低温プラズマ / カーボンナノチューブ / フェロトーシス |
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| Outline of Final Research Achievements |
We obtained fundamental data for the use of combinational therapy of non-thermal plasma (NTP) and carbon nanotubes. We identified hemoglobin, histones and transferrin as specifically adsorbed proteins on carbon nanotube surface with mass spectrometry. Biological effects of NTP exposure were recognized as oxidative stress with biochemical, FACS and immunostaining studies, which was promoted with preincubation with ferric ammonium citrate and repressed by preincubation with redox-inactive iron chelator, desferal. NTP exposure to mesothelioma cells caused an increase in intracellular catalytic Fe(II), lipid peroxidation with increased ferritin/decreased transferrin receptor, which was accompanied by promoted endocytosis and autophagy (ferritinophagy). These data suggest that NTP can cause ferroptosis in mesothelioma cells. As in vitro experiments we also observed that NTP exposure can directly destroy the ferritin core protein and reduce Fe(III) to Fe(II).
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| Free Research Field |
実験病理学
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