Analysis of Non-coding RNA Y1 during iPS reprogramming

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15260
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Gojo Satoshi 京都府立医科大学, 医学(系)研究科(研究院), 教授 (90316745)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: Reprogramming / Non-coding RNA / iPS細胞

Outline of Final Research Achievements

Non coding RNA RNY1 (Y1) is a transiently expressed factor during early iPS reprogramming, and induction efficiency to iPS cells is decreased by its knockdown. We analyzed the molecular machinery related to RNY1 and RO60, which form a complex, during cellular reprogramming. DDX6 protein, which involves in RNA metabolism was identified, and the knockout cell for DDX6 also showed a drastic decrease in induction efficiency. We found that the RNY1/RO60/DDX6 axis attributes to mesenchymal epithelial transition, which is essential for initial phase in iPS reprogramming. and proposed a putative molecular model for parental mRNA decay in the nexus of a set of miRNAs.

Free Research Field

再生医学