Realization of high-throughput artificial chromosome library fabrication technology

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15265
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Mie University
• Principal Investigator: yuda masao 三重大学, 医学系研究科, 教授 (90293779)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: マラリア / 人工染色体

Outline of Final Research Achievements

The malaria artificial chromosome library is an innovative tool that enables genome-wide functional screening of genes and is expected to be applied to a wide variety of post-genomic studies such as identification of drug-resistant genes. However technical difficulties has prevented it from being popularized. For example, it is difficult to analyze multiple samples at the same time because of the complexity of the production processes. The purpose of this research is to a establishe a new technology for producing an artificial chromosome library in a single step by applying the latest transposon technology. This technology realizes genome-wide gene analysis that is not realized even in the model organisms. It will be used in place of NGS in many studies, such as gene identification and gene discovery.

Free Research Field

寄生虫学