Establishing in vitro-in vivo HBV infection system using mouse hepatocytes

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15282
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Osaka University
• Principal Investigator: Ueda Keiji 大阪大学, 医学系研究科, 教授 (00221797)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: HBV / receptor / NTCP / restriction factor / HBV core / mouse hepatocyte / entry

Outline of Final Research Achievements

HBV can not establish the infection in mouse liver cells expressing human NTCP, which is the most important molecule for HBV entry. Since an HBV producing vector transfection into mouse hepatocytes leads to HBV production, there should be some restriction factors to block HBV entry into the cells. In order to clarify the factors in terms of HBV core protein interacting factors, we have established an HBV core protein expressing mouse hepatocyte line.

Free Research Field

ウイルス学