Development of a novel method to reversibly inactivate gene products by using chemical genetics

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15289
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Osaka University
• Principal Investigator: Kurosaki Tomohiro 大阪大学, 免疫学フロンティア研究センター, 特任教授(常勤) (50178125)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ケミカルジェネティクス / 時空間的制御 / Syk / 胚中心 / Cre

Outline of Final Research Achievements

Development of a new method to reversibly inactivate specific genes in a tempo-spatial way is very important for biological research. Here, as a model system, we have tried to reversibly inactivate the Syk tyrosine kinase in the germinal center phase. We have identified the chemical component which inhibits the mutant Syk, but not wildtype Syk. We have also established mice harboring inducible Cre only in the germinal center B cell phase.

Free Research Field

免疫学