The photodynamic detection of MRD of multiple myeloma by flowcytometory

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15324
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Tohoku University
• Principal Investigator: HARIGAE HIDEO 東北大学, 医学系研究科, 教授 (50302146)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 微小残存病変 / フローサイトメトリ― / アミノレブリン酸

Outline of Final Research Achievements

Heme is generated in mitochondria by insertion of iron to protoporphyrin IX (PPIX). As the activity of mitochondria is decreased in cancer cells, PPIX is accumulated by the administration of ALA. The aim of this study is to establish the novel method to detect minimal residual disease (MRD) of multiple myeloma by applying this characteristic of cancer cell biology to flowcytometory. First, the condition to detect the fluorescence of PPIX was established in KMS18 cell, a myeloma cell line. It was confirmed that the established condition can effectively differentiates KMS18 cells from normal blood cells. Next, transporters which incorporate ALA into cells, were identified by the competition assay. Then the movement of ALA after incorporated into cells was clarified using Caco cells. As a result, a part of ALA was used for the generation of PPIX, and unused ALA was exported from cells. These findings ｍay contribute to the development of a novel detection system of MRD.

Free Research Field

血液内科学