Development of rapid qRT-PCR for applying to the gene diagnosis

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15335
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: OBOKI Keisuke 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 副参事研究員 (80415108)
• Co-Investigator(Kenkyū-buntansha): 梶原 直樹 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主任研究員 (70453917) ; 芝崎 太 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, プロジェクトリーダー (90300954)
• Research Collaborator: ENDO Fumiko ; AZUMA Hitomi ; OGAWA Mina ; OYA Yuki ; Daichi Sadato ; YAMAUCHI Hiroshi ; AKAGI Yoshinori ; INUI Nobuhiko
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: PCR

Outline of Final Research Achievements

It was necessary to determine the optimal concentration of the primers for efficient running the high-speed PCR, and it was possible to determine this. In terms of speed, "40 cycles 8 minutes" was achieved. Furthermore, the sensitivity was improved from ~1000 copies to ~100 copies. Finally, we found a solution for the problem of temperature unevenness in the heater of the equipment. However, due to the company collaborator’s explanation that they cannot predict the market size of gene diagnosis in the future, this collaborative research ended, making it impossible to use the equipment. We needed to find a new collaborative research partner, the research subject became pending. It was valuable that we could optimize reagent conditions for the high-speed PCR. Thus, we are looking for the next tactics for the future development.

Free Research Field

腫瘍学