Host responses in whole blood of children infected with respiratory syncytial virus

2016 Fiscal Year Research-status Report

• Project/Area Number: 16K15369
• Research Institution: Tohoku University
• Principal Investigator: Dapat Clyde 東北大学, 医学系研究科, 講師 (10733826)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: RSV / blood / proteins

Outline of Annual Research Achievements

This study aims to identify host responses in whole blood of children infected with respiratory syncytial virus (RSV) using proteomics approach. Approval from ethics committee were obtained at Tohoku University Graduate School of Medicine, Sendai Medical Center, and Miyagi Children’s Hospital. Pilot experiments were successfully performed including collection of peripheral blood samples, isolation and purification of total RNA and proteins.

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

Succeeded in obtaining the approval from the ethics committee of Sendai Medical Center and Miyagi Children's Hospital and performing pilot experiments.

Strategy for Future Research Activity

Enrollment of children infected with respiratory syncytial virus at Sendai Medical Center and Miyagi Children’s Hospital. Collection of blood samples from patients. Isolation and purification of RNA and proteins. Proteome profiling of patients by liquid chromatography-mass spectrometry.

Causes of Carryover

The amount incurred was appropriated for reagents for sample collection and proteomics. Since the approval from ethics committee was obtained in December 2016, we plan to purchase these reagents in 2017.

Expenditure Plan for Carryover Budget

We plan to use this amount to purchase reagents for sample collection and proteomics in 2017.