2018 Fiscal Year Final Research Report
Development of rapid classification method for new drug resistance genotyping by mass spectrometry (MALDI-TOF MS) of Enterobacteriaceae
| Project/Area Number |
16K15393
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical and hospital managemen
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| Research Institution | International University of Health and Welfare |
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Principal Investigator |
NAGASAWA ZENZO 国際医療福祉大学, 福岡保健医療学部, 教授 (80706820)
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| Research Collaborator |
FUNASHIMA yumiko
KATO kyohei
HANAIWA hiroki
NARITA taeko
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | MALDI-TOF MS / ESBL / CPE / ClinPro Tools software / flexAnalysis software |
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| Outline of Final Research Achievements |
The applicant focused on MALDI-TOF MS technology and examined discrimination of Extended spectrumβ-lactamasesEnterobacteriaceae（ESBL） and carbapenemase producing Enterobacteriaceae（CPE）．As a result of analyzing ESBL and CPE with ClinPro Tools and flexAnalysis, both sensitivity and specificity were low in discrimination at highly specific peaks and analysis by various algorithms.
However, in the IUHW2 method originally developed by the applicant, only CPE showed a sensitivity of 82% and a specificity of 70%, suggesting that they are useful for detection. About analysis by this IUHW2 method, we think that further examination is necessary about Pseudomonas aeruginosa and Acinetobacter spp. etc.
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| Free Research Field |
臨床微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究ではESBL産生菌およびCPE産生菌にて得られたマススペクトル波形を利用して迅速に薬剤耐性菌を鑑別する技術の開発を実施した。その結果、既存のClinPro Tools（特異ピーク、各種アルゴリズム解析） やflexAnalysis （特異ピーク）では鑑別が困難であったが、独自に開発した共通ピーク除去解析技術（IUHW2法）ではCPE産生菌のみ感度82％、特異度70％を示し検出に有用性があることが示唆された。このIUHW2法は簡易にコンピュターシステムにより自動的に波形解析が実施できるため、緑膿菌やアシネトバクターなど多くの薬剤耐性菌の鑑別にも応用できる可能性が示唆された。
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