Elucidation of the mechanisms of HBV infection using single cell transcriptome analysis

2016 Fiscal Year Final Research Report

• Project/Area Number: 16K15426
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Kanazawa University
• Principal Investigator: HONDA Masao 金沢大学, 保健学系, 教授 (00272980)
• Co-Investigator(Renkei-kenkyūsha): SHIRASAKI Takayoshi 金沢大学, 保健学系, 助教 (50547180) ; KAWAGUCHI Kazunori 金沢大学, 附属病院, 助教 (90579632)
• Research Collaborator: Oishi Naoki
• Project Period (FY): 2016-04-01 – 2017-03-31
• Keywords: ウイルス / 遺伝子 / 感染症 / 発現制御

Outline of Final Research Achievements

We identified two essential host genes for HBV replication by using single cell transcriptome analysis of KM cells that were established from a HCC tissue derived from a patient with chronic hepatitis B. Repression of two genes reduced cccDNA, and by the combination of ETV, more than 95% reduction of cccDNA was obtained. By using HepG2-NTCP cells in which two genes were knocked down, primary human hepatocytes (PXB) and HepG2-NTCP cells in which two genes were overexpressed by lentivirus system, we showed HBV-DNA, cccDNA and pgRNA were substantially changed by the expression of two genes.

Free Research Field

肝臓病学