Establishment of RNA virus vaccine for removal of pluripotent cells in iPS cell-derivative

2016 Fiscal Year Final Research Report

• Project/Area Number: 16K15448
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: Keio University
• Principal Investigator: Fukuda Keiichi 慶應義塾大学, 医学部(信濃町), 教授 (20199227)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Keywords: 再生医療 / iPS細胞 / 免疫療法 / がんワクチン

Outline of Final Research Achievements

To establish of proof of concept of removal of pluripotent cells from induced pluripotent stem cell-derivatives, we tried to applied human embryonic stem cells to in vivo vaccination. In in vivo experiments of mouse assay, growth of teratoma which was derived from transplanted syngeneic mouse induced pluripotent stem cells were suppressed when mouse was vaccinated with irradiated human embryonic stem cells before transplantation. Additionally, vaccination with human embryonic stem cells completely inhibit teratoma formation when 1x10^5 syngeneic mouse induced pluripotent stem cells were transplanted. These results indicated that immune rejection against antigens which exists in pluripotent stem cells can be applicable to removal of pluripotent cells from induced pluripotent stem cell-derivatives in vivo.

Free Research Field

循環器内科