Visualization of small metabolites in the kidney for elucidation of pathophysiology of ischemic nephropathy

2016 Fiscal Year Final Research Report

• Project/Area Number: 16K15471
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Keio University
• Principal Investigator: Itoh Hiroshi 慶應義塾大学, 医学部(信濃町), 教授 (40252457)
• Research Collaborator: MIYASHITA Kazutoshi 慶應義塾大学, 医学部, 専任講師 (50378759)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Keywords: 質量分析イメージング / アデノシン / ATP / 腎臓

Outline of Final Research Achievements

The kidney plays an essential role in the maintenance of physiological homeostasis of fluid and electrolytes in the body. Adenosine is a well known regulator of renal function and blood flow; however, the normal distribution of adenosine in the kidney has not been well elucidated. Matrix-assisted laser desorption/ionization (MALDI) - imaging mass spectrometry (IMS) is a novel technology for visualizing the distributions of molecules in situ. Small metabolites in the kidney has not been examined by the method thus far. In the present study, we identified a remarkable accumulation of adenosine in outer stripes of outer medulla (OSOM). The local accumulation of adenosine in OSOM may play a significant role for the physiological functions of adenosine such as the regulation of interstitial blood flow in the kidney.

Free Research Field

内分泌学、代謝学、腎臓内科学