Development of drug delivery system to mitochondria and a drug for the treatment of mitochondrial disease

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15482
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Kumamoto University
• Principal Investigator: Tomizawa Kazuhito 熊本大学, 大学院生命科学研究部(医), 教授 (40274287)
• Co-Investigator(Renkei-kenkyūsha): ANDO Yukio 熊本大学, 大学院生命科学研究部, 教授 (20253742)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ミトコンドリア / DDS / 糖尿病 / ミトコンドリア病 / リポソーム

Outline of Final Research Achievements

We generated the peptides consisting of mitochondrial localizing signal peptide derived from Cdk5rap1 and poly arginine (9R). We incubated primary cultured myocardial cells, skeletal muscle cels and neurons with the peptides. The peptides were localized on mitochondria. Moreover, we generated the liposomes containing eperisone, which are conjugated with the mitocondrial signal peptide. When applied the liposomes to Cdk5rap1-deficient skeletal muscle cells, the liposomes were localized in mitochondria and mitochondrial functions such as compel activities, mitochondrial membrane potential and the translation of mitochondrial proteins were improved.

Free Research Field

生理学