Functional screening of glycan-related molecules in feedback regulation of hematopoietic stem cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15505
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Soka University
• Principal Investigator: Nishihara Shoko 創価大学, 理工学部, 教授 (00164575)
• Research Collaborator: 伊藤 和義 創価大学, 工学研究科生命情報工学専攻, 大学院生 ; 佐藤 大一 創価大学, 工学研究科生命情報工学専攻, 大学院生 ; 符 敦賢 創価大学, 工学研究科生命情報工学専攻, 大学院生
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 造血器官 / ショウジョウバエ / 糖鎖関連遺伝子 / 成熟血球細胞 / 造血幹細胞 / フィードバック機構

Outline of Final Research Achievements

In order to maintain tissues and organs, their constituent cell types and numbers must be strictly controlled. Therefore, tissue stem cells are under the feedback regulation from mature differentiated tissue cells. However, its mechanism is largely unknown. Here we performed screening of the glycan-related genes that contribute to the feedback regulation in tissue stem cells by using Drosophila hematopoietic organs.
Drosophila lymph gland in 3rd instar larva is a hematopoietic organ, in which hematopoietic stem cells proliferate and mature hemocytes differentiate. To screen, we use RNA interference based on GAL4-UAS system. The knock down of glycan-related genes in mature hemocytes, and then hematopoietic stem cells population were observed.

Free Research Field

糖鎖生物学