Establishment of infection diagnostic and control methods based on disease-related factors using LC-MS / MS and nanodevices

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15518
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Infectious disease medicine
• Research Institution: Osaka University
• Principal Investigator: Yamasaki Seiji 大阪大学, 産業科学研究所, 助教 (70757301)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 感染症 / 排出ポンプ / 細菌 / 抗生物質 / トランスポーター / 多剤耐性菌 / 薬剤耐性 / 抗菌薬

Outline of Final Research Achievements

As a result of this research, as techniques for identifying the physiological substrate of bacterial drug efflux pump, I established simple and rapid methods using various fluorescent substrates.
Through research on bacterial drug efflux pumps utilizing this technique, I elucidated the third route excreting cationic planar aromatic small molecules.
In addition, I finded out drug efflux pump inhibitor candidates involved in bacterial pathogenicity by using inhibitor screening method (Japanese Patent Application No. 2016-028653). These are useful for treatment of infectious diseases.

Free Research Field

細菌学