Challenge for establishing tools to knock out 5-HT receptor genes using CRISPR/Cas9

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15552
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Psychiatric science
• Research Institution: Hokkaido University
• Principal Investigator: Ohmura Yu 北海道大学, 医学研究科, 助教 (80597659)
• Co-Investigator(Kenkyū-buntansha): 吉田 隆行 北海道大学, 医学研究院, 助教 (60374229)
• Co-Investigator(Renkei-kenkyūsha): YAMANAKA Akihiro 名古屋大学, 環境医学研究所, 教授 (60323292)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ゲノム編集 / セロトニン / 体温

Outline of Final Research Achievements

Because there are 14 subtypes of 5-HT receptor, conventional gene manipulations will require us to pay too much cost for examining all the subtypes. The purpose of this study was to establish a low-cost method for knocking out 5-HT receptor genes by injecting virus vector to the brain of Cas9-expressing mice. The hypothermic effect of serotonin 5-HT1A receptor agonist was attenuated in virus-injected mice. Analysis using DNA mismatch cleavage enzyme confirmed mutation of 5-HT1A receptor gene. However, we failed to obtain electrophysiological evidence using patch clamp methods because the condition of cells was not good.

Free Research Field

精神薬理学