2017 Fiscal Year Final Research Report
Challenge for establishing tools to knock out 5-HT receptor genes using CRISPR/Cas9
Project/Area Number |
16K15552
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Psychiatric science
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Research Institution | Hokkaido University |
Principal Investigator |
Ohmura Yu 北海道大学, 医学研究科, 助教 (80597659)
|
Co-Investigator(Kenkyū-buntansha) |
吉田 隆行 北海道大学, 医学研究院, 助教 (60374229)
|
Co-Investigator(Renkei-kenkyūsha) |
YAMANAKA Akihiro 名古屋大学, 環境医学研究所, 教授 (60323292)
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Keywords | ゲノム編集 / セロトニン / 体温 |
Outline of Final Research Achievements |
Because there are 14 subtypes of 5-HT receptor, conventional gene manipulations will require us to pay too much cost for examining all the subtypes. The purpose of this study was to establish a low-cost method for knocking out 5-HT receptor genes by injecting virus vector to the brain of Cas9-expressing mice. The hypothermic effect of serotonin 5-HT1A receptor agonist was attenuated in virus-injected mice. Analysis using DNA mismatch cleavage enzyme confirmed mutation of 5-HT1A receptor gene. However, we failed to obtain electrophysiological evidence using patch clamp methods because the condition of cells was not good.
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Free Research Field |
精神薬理学
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