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2017 Fiscal Year Final Research Report

Establishment of a system to measure DNA repair activity using circulating tumor cells: the key to predict sensitivity to PARP inhibitors

Research Project

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Project/Area Number 16K15594
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General surgery
Research InstitutionNagasaki University

Principal Investigator

MITSUTAKE Norisato  長崎大学, 原爆後障害医療研究所, 准教授 (50404215)

Co-Investigator(Kenkyū-buntansha) 中沢 由華  名古屋大学, 環境医学研究所, 助教 (00533902)
Research Collaborator TANAKA Aya  
Project Period (FY) 2016-04-01 – 2018-03-31
Keywords乳癌 / DNA修復
Outline of Final Research Achievements

We have developed a method to isolate and proliferate primary breast cancer cells from clinical tissue samples. Using this method, we have made frozen stocks of the cells isolated from cases with high probability of familial/hereditary breast cancers.
Although isolating circulating tumor cells (CTCs) was possible, it was unfortunately difficult to propagate them. Because of that, we focused on the study using cells isolated from cancer tissues.
We have developed a method by which DNA repair activity by homologous recombination can be measured. In this method, residual DNA damage was quantified at the G2 phase using nuclear and phospho-H2AX staining.

Free Research Field

内分泌腫瘍学

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Published: 2019-03-29  

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