2017 Fiscal Year Final Research Report
Single cell heterogeneity analysis of endothelial and mesenchymal cells
| Project/Area Number |
16K15597
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Keywords | iPS細胞 / 再生医療 / シングルセルゲノミクス |
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| Outline of Final Research Achievements |
Comprehensive gene expression analysis was performed on human umbilical cord-derived vascular endothelial cells and human bone marrow mesenchymal cells at the single cell level. Gene expression data on vascular endothelial cells and mesenchymal cells in the iPSC- liver bud was also obtained. Genes expressed in each cell population were analyzed. Furthermore, it was revealed that genes involved in cell-cell interaction in iPSC liver bud. We also analyzed heterogeneity in each cell population. These findings are useful information for quality evaluation in the process of manufacturing human iPSC- liver bud, and for future improvement of iPSC- liver bud quality and safety.
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| Free Research Field |
幹細胞生物学
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