Development of genetically modified T cell transfer therapy targeting peripheral circulating cancer cells of solid tumor

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15602
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Kanagawa Cancer Center Research Institute
• Principal Investigator: wada satoshi 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), がんワクチンセンター, 医長 (30420102)
• Co-Investigator(Kenkyū-buntansha): 矢田 英理香 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), その他部局等, その他 (30415567)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 腫瘍免疫 / 循環がん細胞 / CTC / pancreatic cancer

Outline of Final Research Achievements

CTC analysis using patient samples has been carried out in 25 patients and CTC could be confirmed using blood circulation cancer cell concentration device. In immunostaining experiments, staining was perfomed using epithelial marker (cadherin) and hematopoietic cell marker (CD45), and cadherin (+) CD45 (-) cells were counted as CTC (before treatment: 0-312 cells / 1 sample, after treatment: 0-150 cells / 1 sample). In the PDX model, conditions were examined using a sample in which mouse blood and human pancreatic cancer cells were mixed. Furthermore, in the CDX model using the pancreatic cancer cell line, peripheral blood was collected at the time when tumor volume reached 1500 mm3, and CTC cells (EGFP (-), mouse MHC (-), HLA (+)) were identified. After these fundamental analysis, we performed a similar experiment using pancreatic cancer PDX model and succeeded in identifying CTC cells.

Free Research Field

腫瘍免疫