analysis of calcification process in implanted arterial artificial-graft.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15632
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: The University of Tokushima
• Principal Investigator: KUROBE Hirotsugu 徳島大学, 大学院医歯薬学研究部(医学系), 助教 (30380083)
• Co-Investigator(Kenkyū-buntansha): 吉田 守美子 徳島大学, 大学院医歯薬学研究部, 講師 (40510904)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 生体分解性ポリマー / 血管移植 / 血管再生 / 石灰化 / 遺伝子発現解析

Outline of Final Research Achievements

Purpose of his study clarify the of calcification process in implanted arterial artificial-graft. Artificial arterial graft was implanted to abdominal aorta in 49 mice, and we explanted the implanted grafts from 39 mice at 2 months after implantation. By X-ray analysis, 14 explanted grafts occurred calcification. We divided 2 groups; Group C in 14 grafts with calcification, and Group N is 25 grafts without calcification.
By microarray analysis with both groups, we picked up genes which was more than 2 fold in Fold Change analysis. And, by real time PCR was done in these picked up 22 genes, we think 4 genes (collagen X、osx、win3a、ankrd2) were associated with calcification process. We are doing more analysis to clarify the relation of these 4 genes for calcification process after graft-implanting.

Free Research Field

医歯薬学