2017 Fiscal Year Final Research Report
Elucidation for Histone induced cell death in sepsis
| Project/Area Number |
16K15681
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山崎 創 東邦大学, 医学部, 准教授 (70315084)
中野 裕康 東邦大学, 医学部, 教授 (70276476)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKANO Hiroyasu 東邦大学, 医学部, 教授 (70276476)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | Histone / sepsis / cell death / gene trap |
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| Outline of Final Research Achievements |
Circulating histones are major mediators of sepsis and cytotoxic to vascular endothelium. For analyzing cytotoxic mechanism of histones, we constructed a haploid genetic screen system using HAP1 cells. Infection efficiency of gene-trap vector to HAP1 cells was 32%, it revealed that high frequency integration was occurred in gene structures. We treated gene-trapped HAP1 cells with histones, surviving cells were treated with histones again.
We could not find a mediator gene of histone toxicity, but histones have possibilities medical targets for sepsis.
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| Free Research Field |
分子細胞生物学
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