2017 Fiscal Year Final Research Report
Differentiation of Leydig cells
| Project/Area Number |
16K15688
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Kobe University |
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Principal Investigator |
Ishida Takaki 神戸大学, 医学部附属病院, 医員 (10771850)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | leydig細胞 / ヒトiPS細胞 / テストステロン / NR5A1 / 17βHSD3 / LHCGR / アルドステロン / コルチゾール |
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| Outline of Final Research Achievements |
we transduced NR5A1 expressing system by doxycycline addition to human iPS cell. NR5A1 forced expression iPS cells differentiate into Human iPS cells-derived testosterone-producing Leydig cells. Leydig cell marker (INSL3 variant2) and LH receptor (LHCGR) and Leydig cell-specific enzyme（17βHSD3）and expression of various enzyme (StAR,CYP11A,3βHSD2,CYP17A) necessary for testosterone production were confirmed in RT-PCR.INSL3 and LHCGR were confirmed in immunohistochemical staining. Testosterone production was confirmed by the hormone measurement in supernatants.
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| Free Research Field |
泌尿器
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